FIG. 3.

Black lipid bilayer analysis. Single-channel conductance measurements after addition of BrkA C-terminal protein that had been further purified by elution from an SDS-PAGE gel. Protein was added to a 1 M KCl solution bathing a membrane of 1.5% oxidized cholesterol in n-decane. There was an applied voltage of 50 mV. The arrows indicate stepwise increases in conductance.