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Black lipid bilayer analysis. Single-channel conductance measurements were done after addition of first protein from a clone containing the vector alone (JS13) and then the BrkA C-terminal protein (RF1065). Protein was added to a 1 M KCl solution bathing a membrane of 1.5% oxidized cholesterol in n-decane. There was an applied voltage of 50 mV. The protein from JS13 was purified in the same way as the protein from RF1065.
