Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Schizosacchoromyces pombe) | mCherry-Psy1, Ish1-GFP | This manuscript | FC3318 | h- ade6 <<mCherry-psy1 ish1-GFP:kanMX ura4-D18 |
| Genetic reagent (S. pombe) | gpd1 mutant, mCherry-Psy1, Ish1-GFP | This manuscript | FC3290 |
h- ade6 <<mCherry-psy1 ish1-GFP:kanMX gpd1::hphMX6
ura4-D18 ade6- |
| Genetic reagent (S. pombe) | gpd1 mutant | This manuscript | FC3291 | h- gpd1::hphMX6 ade6-M216 leu1-32 ura4-D18 his3-D1 |
| Genetic reagent (S. pombe) | mCherry-Psy1, Cut11-GFP | This manuscript | FC3319 |
h? cut11-GFP:ura4 +ade6:mCherry-psy1
ura4-D18 leu1-32 ade6-M210 |
| Genetic reagent (S. pombe) | gpd1 mutant, CytGEMs | This manuscript | FC3320 | h- gpd1::hphMX6 pREp41X-Pfv-Sapphire leu1-32 ade6- leu1-32 ura4-D18 his7-366 |
| Genetic reagent (S. pombe) | gpd1 mutant, NucGEMs | This manuscript | FC3321 | h- gpd1::hphMX6 pREp41X-NLS-Pfv-Sapphire leu1-32 ade6- leu1-32 ura4-D18 his7-366 |
| Genetic reagent (S. pombe) | CytGEMs | This manuscript | FC3289 | h- pREp41X-Pfv-Sapphire ade6-M216 leu1-32 ura4-D18 his3-D1 |
| Genetic reagent (S. pombe) | NucGEMs | This manuscript | FC3322 | h- pREp41X-NLS-Pfv-Sapphire leu1-32 ade6- leu1-32 ura4-D18 his7-366 |
| Genetic reagent (S. pombe) | pom1 mutant, mCherry-Psy1, Ish1-GFP | This manuscript | FC3323 | h- pom1::ura4 ade6 <<mCherry-psy1 ish1-GFP:kanMX |
| Genetic reagent (S. pombe) | Rpl3001-GFP | Chang Lab collection | FC3215 | h+rpl3001-GFP:kanR leu1-32 ura4-D18 ade6-210 |
| Genetic reagent (S. pombe) | Rpl2401-GFP | Chang Lab collection | FC3213 | h- rpl2401-GFP:kanR leu1-32 ura4-D18 ade6-216 |
| Genetic reagent (S. pombe) | Rps2-GFP | Chang Lab collection | FC3209 | h- rps2-GFP:kanR leu1-32 ura4-D18 ade6-210 |
| Genetic reagent (S. pombe) | 1XE2C, GFP-Psy1 | This manuscript | FC3324 | h+act1p:1XE2C:HygR leu2:GFP-psy1 leu1- ura4-D18 his7-366 |
| Chemical compound/drug | YES 225 Media | Sunrise Science Production | #2011 | |
| Chemical compound/drug | Edinburgh Minimum Media (EMM) | MP Biomedicals | #4110–32 | |
| Chemical compound/drug | Histidine | Sigma-Aldrich | #H8000 | |
| Chemical compound/drug | Uracil | Sigma-Aldrich | #U0750 | |
| Chemical compound/drug | Adenine | Sigma-Aldrich | #A9126 | |
| Chemical compound/drug | Thiamine | Sigma-Aldrich | #T4625 | |
| Chemical compound/drug | Lallzyme | Lallemand | #EL011-2240-15 | |
| Chemical compound/drug | Leptomycin B (LMB) | Alfa Aesar | #87081-35-4 | |
| Chemical compound/drug | Ethanol | Fisher BioReagents | #BP2818-500 | |
| Chemical compound/drug | Dimethyl sulfoxide (DMSO) | Fisher Scientific | #67-68-5 | |
| Chemical compound/drug | Cycloheximide (CHX) | Sigma-Aldrich | #C7698 | |
| Chemical compound/drug | Agarose | Invitrogen | #16500500 | |
| Chemical compound/drug | 4% formaldehyde (methanol-free) | Thermo Scientific | #28,906 | |
| Chemical compound/drug | RNAse | Thermo Scientific | #EN0531 | |
| Chemical compound/drug | Fluorescein isothiocyanate isomer I (FITC) | Sigma | #F7250 | |
| Software, algorithm | µManager v. 1.41 | Edelstein et al., 2010; Edelstein et al., 2014 | ||
| Software, algorithm | Matlab | Mathworks | R2018b | |
| Software, algorithm | Python | Drake Jr and Van Rossum, 1995 | 5.5.0 | |
| Software, algorithm | Prism | GraphPad | Version 9.3.1 | |
| Software, algorithm | FIJI ImageJ | Schindelin et al., 2012 | ||
| Other | µ-Slide VI 0.4 channel slide | Ibidi | #80,606 | microfluidic chambers |
| Other | µ-Slide VI 0.5 glass bottom channel slides | Ibidi | #80,607 | microfluidic chambers |