Skip to main content
. 2022 Aug 11;13:964564. doi: 10.3389/fmicb.2022.964564

Table 3.

Comparison of significantly changed rumen metabolites in three treatments.

Superclass Metabolite names Treatmentsa SEMb q-values
C50 C65 C80 Treatment Linear Quadratic
Lipids and lipid-like molecules LPC 18:1 194691104.7 42708696.68 73549354.12 27916602.05 0.049 0.057 0.056
(+/−)14(15)-DiHETE 7896496.452 6041559.384 10162068.62 673300.6846 0.028 0.113 0.038
PE (18:0/18:1) 568223637.8 162,546,434 310528017.1 55846937.5 0.002 0.013 0.002
Oleanolic acid 10860029.05 25271500.23 31586962.46 3021353.7 0.005 0.002 0.165
Docosanoic acid 120465591.8 312561086.8 278834439.5 29895781.66 0.007 0.011 0.014
FAHFA (18:0/20:2) 298961990.2 196,149,233 131557819.5 22268756.13 0.001 <0.001 0.192
LPS 19:0 38712801.23 26590129.57 17910065.26 2915932.416 0.003 0.001 0.325
1a,1b-Dihomo prostaglandin E1 6101762.044 10267200.84 15370526.54 1314266.816 0.004 0.001 0.728
9-KODE 5199482.404 10792441.07 14612730.08 1337414.414 0.004 0.001 0.315
FAHFA (16:0/19:2) 14935684.43 13776392.47 6942936.672 1166944.149 0.002 0.001 0.271
8-Isoprostaglandin E2 7185522.319 7089838.662 2292831.814 802492.4605 0.006 0.004 0.175
5-Phenylvaleric Acid 4951271.466 2543348.639 2308797.869 403568.0036 0.003 0.002 0.029
Ursolic acid 67370520.13 262907645.3 329005364.6 43450056.48 0.023 0.009 0.209
PC (17:2/18:5) 10851812.01 3702900.431 3488810.378 1207361.893 0.006 0.004 0.033
(±)12(13)-DiHOME 1,108,505,784 557158819.6 1,438,674,449 148732595.2 0.035 0.289 0.024
FAHFA (20:0/22:3) 3800547.937 4999730.565 3051165.382 356261.5261 0.067 0.339 0.044
FAHFA (20:0/20:0) 2552261.78 3927179.497 2148783.717 298715.7078 0.024 0.499 0.011
FAHFA (20:1/22:3) 1225762.082 3295555.767 1397935.437 296420.9791 0.001 0.688 0.000
octadec-9-ynoic acid 142275777.6 119237853.7 358769046.8 35729092.27 0.002 0.002 0.054
Methyl dihydrojasmonate 39830838.48 25142191.38 55470567.17 4440973.719 0.008 0.069 0.011
Organic acids and derivatives 4-Acetamidobutanoic acid 339558459.3 705539699.7 614883543.4 83776799.16 0.183 0.178 0.145
Glycohyocholic acid Sodium salt 1121650.667 2160565.954 1293457.42 171128.2522 0.015 0.6 0.004
Glycochenodeoxycholic acid sodium salt 138001.0444 102694.7378 210195.6813 27122.37049 0.273 0.284 0.290
N6,N6,N6-Trimethyl-L-lysine 22992800.87 49865733.38 43396522.91 3542298.6 <0.001 0.001 0.025
3-(1-cyano-1,2-dihydroisoquinolin-2-yl)-3-oxopropyl propionate 9809639.689 5114501.201 3930670.708 847991.9981 0.002 0.001 0.056
2-Phenylglycine 8903501.364 12633774.31 26737460.27 2435616.441 0.001 <0.001 0.349
FLK 4400166.146 2468044.319 1353037.499 397453.436 0.001 <0.001 0.133
L-Cystathionine 1626295.198 1518461.996 935345.7416 109842.2172 0.009 0.004 0.395
Val-Ser 74101368.05 56742398.55 35016005.23 5274132.354 0.002 <0.001 0.712
Gly-Tyr-Ala 10585113.74 5431686.462 3484016.941 1071800.745 0.007 0.003 0.157
Methylmalonic acid 6,854,758,528 10,232,838,938 6,095,994,035 644249580.7 0.008 0.517 0.003
D-Proline 8790839.633 21238605.66 12225921.38 1605430.561 <0.001 0.122 0.000
Methyl-2-hydroxyisobutyric acid 5627003.487 11841335.87 6323869.199 993122.1649 0.007 0.696 0.002
5-S-cysteinyldopa 359632.8043 1151730.365 622027.8277 102360.9832 <0.001 0.082 0.000
Organoheterocyclic compounds Uracil 2,921,751,171 2,509,346,257 2,244,999,054 152680217.3 0.196 0.079 0.617
Roquefortine C 121793862.4 69710112.4 44521541.9 12,205,993 0.016 0.006 0.272
Vitamin B2 9657353.128 8023761.343 5587224.07 626803.3111 0.014 0.004 0.918
6-Dimethylaminopurine 44812097.53 20080013.45 16138724.04 4571946.438 0.008 0.004 0.072
Organic oxygen compounds D-(+)-Mannose 1,220,583,273 2,446,884,559 1,865,896,826 184868124.8 0.012 0.08 0.006
L-Threonic acid 75199858.64 153399194.6 83824327.61 14171561.31 0.032 0.765 0.010
D-Galactosamine 3924881.77 9228256.303 6480804.621 697657.3705 0.001 0.029 0.000
L-Kynurenine 7186761.226 8638010.59 9452862.755 354037.1177 0.016 0.005 0.320
L-(−)-Glyceric acid 241945895.5 324209480.6 188479467.4 21370398.42 0.018 0.211 0.014
Benzenoids 4-Hydroxybenzoic acid 18829863.68 11260717.01 11002717.31 1431899.843 0.025 0.016 0.082
Isohomovanillic acid 49367075.51 26081257.83 39488821.6 4000030.654 0.044 0.249 0.018
Homovanillic acid 21776146.29 18347209.78 15121282.09 922797.1842 0.003 0.001 0.432
2-[(carboxymethyl)(methyl)amino]-5-methoxybenzoic acid 106638622.4 324209480.6 188479467.4 21370398.42 0.085 0.321 0.060
Nucleosides, nucleotides, and analogues 2’-O-Methylguanosine 102175235.9 172895328.6 115928891.7 12413194.3 0.033 0.588 0.010
1-Methylguanosine 13592217.39 17438884.08 5796441.008 1554608.446 0.001 0.005 0.004
Pseudouridine 70207322.22 143837465.1 74685038.63 11250678.16 0.002 0.806 0.001
Organic nitrogen compounds Spermidine 229394701.5 85090134.18 46356478.24 27157907.31 0.004 0.002 0.081
2,6-di(2-thienylmethylidene)cyclohexan-1-one 92684266.52 47489798.42 47789129.5 6922044.883 0.001 0.001 0.009
Phenylpropanoids and polyketides LSD-d3 29688333.54 19809762.59 17268311.85 1820801.817 0.003 0.001 0.068
3-hydroxy-3,4-bis[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-2-one 1912453.091 1004142.123 1065828.76 127195.7192 <0.001 <0.001 0.002
Alkaloids and derivatives 6-Acetylmorphine 12992029.47 7645667.857 5767021.69 1116835.001 0.01 0.004 0.160
a

C50, diet contained 50% of concentrate; C65, diet contained 65% of concentrate; C80, diet contained 80% of concentrate.

b

SEM, standard error of the mean. Significance considered at p < 0.05.