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. 2022 Aug 24;42(34):6654–6666. doi: 10.1523/JNEUROSCI.0228-22.2022

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Copyright © 2022 Marchionni, Pilati et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Inhibitory synaptic transmission between L2/3 SOM INs and L2/3 PCs is unaltered in FHM1 mice. A, Left, Schematic diagram of the experimental configuration in which APs were elicited in a presynaptic L2/3 SOM IN in current clamp (I-clamp; −70 mV) and the uIPSCs evoked by the APs were recorded in a connected postsynaptic L2/3 PC in voltage clamp (V-clamp; 0 mV); representative traces of a presynaptic AP and postsynaptic uIPSCs are also shown. A representative epifluorescence image of an EGFP-positive SOM IN and a bright field image of a L2/3 PC after biocytin staining are shown on top of the diagram. Right, Time course of a representative experiment showing the uIPSCs evoked in a L2/3 PC before and after the application of a saturating concentration of the specific Ca_V2.1 inhibitor ω-agatoxin-IVA (Aga; 400 nm) in WT mice. Inset, Mean uIPSC before (black) and after (red) bath application of Aga. Right, Percentages of inhibition of the mean uIPSC after application for 17 min of Aga (average value: 98 ± 2%; n = 5, N = 5). B, Left, Representative uIPSCs (top) evoked in a L2/3 PC and corresponding mean uIPSC (bottom) in WT (black) and FHM1 (red) mice. Right, Amplitudes of the mean uIPSC in L2/3 PCs in WT (average value: 61 ± 15 pA, n = 15, N = 10) and FHM1 (average value: 62 ± 11 pA, n = 15, N = 13) mice. The average values of the mean uIPSC elicited in a L2/3 PC by APs in a connected presynaptic SOM IN are similar in FHM1 and WT mice (f₍₂₈₎ = 0.44, p = 0.59 MW test). C, Left, Percentages of failures in WT (average value: 7.5 ± 3.0%, n = 15, N = 10) and FHM1 (average value 4.1 ± 2.4% n = 15, N = 13) mice. Right, Paired pulse ratios (mean uIPSC2/mean uIPSC1; 50-ms interval) in WT (average value: 0.92 ± 0.04, n = 15, N = 10) and FHM1 (average value 0.85 ± 0.03, n = 15, N = 13) mice. Inset, Average mean uIPSCs elicited by a paired pulse in WT (black) and FHM1 (red) PCs. The percentage of failures and the paired pulse ratio are both similar in FHM1 and WT mice (f₍₂₈₎ = 0.38, p = 0.16 MW test and t₍₂₈₎ = 1.36, p = 0.19 t test, respectively).