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. Author manuscript; available in PMC: 2022 Aug 25.
Published in final edited form as: Exp Cell Res. 2018 May 4;368(2):225–235. doi: 10.1016/j.yexcr.2018.05.001

Fig. 2. Up regulation of GABAergic axis.

Fig. 2

a) HeatMap of GABAergic pathway genes; for each gene, log ratio and related p-values are shown. b) Validation of RNAseq data on neurons from and independent differentiation experiment demonstrate a significant upregulation of GAD1 mRNA and protein levels and of GABA-R receptor alpha 1 (GABA-AR) protein in MECP2-mutated neurons compared to controls. GAPDH was used as loading control for western blot analysis. n=3. c) Upregulation of GRIN2B mRNA in neurons from the 3 MECP2-mutated clones respect to neurons from the partial isogenic control clone (2271#2). Statistical significance was determined using unpaired student’s t test (*p<0.05; **p<0.001; ***p<0.0001).