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. 1999 Sep;181(18):5843–5846. doi: 10.1128/jb.181.18.5843-5846.1999

FIG. 1.

FIG. 1

Complementation of E. coli fur strain H1780 for in vivo repression of fiu::lacZ gene fusion by B. japonicum library clones. (A) B. japonicum genomic region represented by library plasmids pSKBJF1, pSKBJF9, and pSKBJF22, which complemented the fur strain on plates as described in the text. pSKBJF800 is an ApaI-Sau3AI-digested subclone of pSKBJF22. The arrow represents the open reading frame encoding the fur homolog. Restriction sites: A, ApaI; B, BglII; C, ClaI; E, EcoRI; Sa, SacI; S, SphI; St, StyI. (B) Repression of β-galactosidase activity in fur strain H1780 by B. japonicum library clones pSKBJF1 (F1), pSKBJF9 (F9), pSKBJF22 (F22), and pSKBJF800 (F800). Cells containing the library vector pBluescript SK(+) (pSK) were the control for unrepressed activity. The positive control was pMH15fur (EcFur), which contained the E. coli fur gene. Also shown is the result for B. japonicum irr gene on pSKSBIrr (Irr).