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. 1999 Sep;181(18):5843–5846. doi: 10.1128/jb.181.18.5843-5846.1999

FIG. 3.

FIG. 3

Metal-dependent binding of BjFur to the Fur box consensus sequence. Gel mobility shift assays were carried out in the presence (A) or absence (B) of 100 μM MnCl2. The DNA used in the assays was radiolabeled with 32P and contained the Fur box consensus sequence 5′-GATAATGATAATCATTATC-3′. The protein samples used were extracts of E. coli fur strain H1780 containing plasmids pBluescript SK(+) (pSK), pMH15fur (EC-Fur), pSKBJF800 (BJ-Fur), or pSKSBIrr (Irr). The DNA was also run in the absence of extract (Free).