Figure 2.

DEX activates the ferroptotic pathway in SIOP. (a) Quantification of the protein level of MT by ELISA. (b) Images of micro-CT. (c) BMD(g/cm³). (d) BV/TV (%). (e) Images of immunofluorescence double staining of CD90 and GPX4 in bone tissues. (f) Quantitative analysis of the area of GPX4/CD90-positive stains. (g) IHC staining of system xc-, GPX4, FSP1, and MT1, and the IHC-positive cells were marked with black arrows. (h–k) Quantitative analysis of the number of the IHC-positive cells in (g). These studies were performed at least 3 biological replicates. Data represent mean ± SD (n = 5). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.005 compared with control group.