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. 2022 Aug 10;50(15):8566–8579. doi: 10.1093/nar/gkac662

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — High concentrations of aminoglycosides do not induce AgR cassettes. (A) Induction ratio of a subset of AgR cassettes in the presence of 128 μg/ml of kanamycin (blue dots). As a reference, the IR values at 0.5 μg/ml of kanamycin from Figure 5 are also represented (green dots). (B) Induction ratio (calculated as the ratio of fluorescence/OD₆₀₀ with kanamycin over fluorescence/OD₆₀₀ without kanamycin) of these strains along their growth curve (18 h). Color code is as in (A). Green: pMBA; red: pMBA_5′-_aacA5; blue: control strains; mauve: strains with identical 5′-UTRs; gray: rest of strains.