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In vitro translation of CGG repeats support frameshifting. (A) In vitro transcribed RNAs (black) used for reconstituted in vitro translation assay (expected amino acid sequence in red). (B) Quantification of in-frame versus frameshifted products in vitro translation assay (n = 3) (polyR-CGG versus polyG-GGC: P = 0.0482; polyR-CGG versus polyA-GCG: P = 0.0423; polyA-GCG versus polyG-GGC: P = 0.9680). (B) Two-sided unpaired Student's t-test. Graphs are mean ± sem. *P< 0.05.
