FIG. 1.

Genetic organization of the region required for the biosynthesis of CS5 pili in ETEC O115:H40. ORFs belonging to the CS5 cluster are designated csfA to csfF. The region is flanked by a variety of IS elements. +, region homologous to part of orfB in Tn21; ∗, region homologous to part of IS66; #, region homologous to part of IS911. IS1 and a defective IS30 are indicated. The upper line refers to the position of the genes relative to the sequence length, in kilobases, with relevant restriction sites indicated. Plasmids used in this study and the ORF(s) they encompass are indicated.