FIG. 2.
T7 RNA polymerase promoter expression of CsfB, CsfC, CsfD, CsfE, and CsfF clones in pBS-SK+. Proteins were labelled with [35S]methionine, and whole-cell fractions on 15% polyacrylamide gels were subjected to gel electrophoresis in sodium dodecyl sulfate, followed by autoradiography of the dried gels. Lane 1, pGEM7-ZF+; lane 2, pPM5631; lane 3, pBS-SK+; lane 4, pPM5390 (CsfF); lane 5, pPM5636 (CsfC); lane 6, pPM5605 (CsfB); lane 7, pPM5633 (CsfE); lane 8, pPM5640 (CsfD). Arrows indicate the positions of the overexpressed proteins.