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. 2022 Aug 18;2022:1435173. doi: 10.1155/2022/1435173

Figure 2.

Figure 2

Silencing of MYC inhibits glycolysis in HCC cells. (a) The transfection efficiency of si-MYC in MHCC97H and Huh7 cells measured using western blot. The changes in glucose consumption (b), lactate production (c), and ATP levels (d) in MHCC97H and Huh7 cells measured by their respective kits. (e) The protein expression of HK1 and HK2 in MHCC97H and Huh7 cells assessed using Western blot. (f) The proliferation of MHCC97H and Huh7 cells measured using CCK8. (g) Detection of cell proliferation by trypan blue assay. (h) The aggressiveness of MHCC97H and Huh7 cells examined using Transwell assays. p < 0.05 vs. si-NC. Statistical results were presented by mean ± SD. Unpaired t-test and one-way or two-way ANOVA were used for comparisons. Each reaction was run in triplicate.