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. 2022 Aug 26;106(18):5863–5877. doi: 10.1007/s00253-022-12135-2

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© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 3 — Challenges that needs to be resolved before PAC-MAN could be used therapeutically. These challenges include a need suitable delivery tool, immunologic effects of cas proteins, interfere of secondary structure of RNA and expression