Fig. 1.

Preparation of DM modified 3D printed PCL scaffold. (a)3D printed PCL scaffold. Scale bar 500 μm. (b) Isolated BMSCs and osteocytes growing on dish. Scale bar 20 μm. (c)SEM images of scaffolds un-decellularized and decellularized. Scale bar 50 μm. (d) Hochest 33258 staining for cell nucleus before and after un-decellularized and decellularized. Scale bar 100 μm. (e–g) DNA, collagen, and GAGs quantitation of the scaffolds un-decellularized and decellularized. BMSC, bone marrow stromal cell; WTO, wild-type osteocyte; daCO, osteocyte with dominant active β-catenin. Images and data are representative of n = 3 individual experiments. Data were expressed as mean ± SD. *p < 0.05 v.s. un-decellularized by one way ANOVA.