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. 2022 Aug 16;21:110–128. doi: 10.1016/j.bioactmat.2022.07.017

Fig. 2.

Fig. 2

Biocompatibility assay of DM modified 3D printed PCL modules. (a) Live-Dead staining to analyze the viability of BMSCs on DM modified modules for 24 h, live cells (green) and dead cells (red). Scale bar 250 μm. (b) ImageJ analysis of the vitality of BMSCs on DM modified modules. (c) Cytoskeleton staining of BMSCs cultured on DM modified modules and none-DM scaffolds after 24 h. Scale bar 50 μm. (d) ImageJ analysis of cell speading area. (e) CCK-8 analysis the proliferation of cells on DM modified modules and none-DM scaffolds on day 1,4,7. (f) Cytoskeleton staining and (g) AP staining of BMSCs cultured on four groups scaffolds with/without Cyto D. (h) Gene expressions of Alpl, Runx2, Col1a1, and Bglap after 7 days of culture. DM, decellularized matrix; none-DM, PCL; BMSC, bone marrow stromal cells; WTO, wild-type osteocytes; daCO, osteocyte with dominant active β-catenin. Images and data are representative of n = 3 individual experiments. Data were expressed as mean ± SD. *p < 0.05 v.s. none-DM;#p < 0.05 v.s. BMSC-DM, $p < 0.05 v.s. WTO-DM by one way ANOVA. Multiple influencing factors analysis by two way ANOVA.