Fig. 5.

Osteoblast activity in daCO-DM-induced bone. After implantation 4 wk (a) and 8 wk (b), picrosirius red staining showed type I collagen was bright red or yellow under light microscope (LM) and yellow or red, closely arranged and strong birefringence under polarized light microscope (PLM). Scale bar 50 μm. Sections with newly formed bone were measured for each mouse and 5 mice were analyzed for each group. DM, decellularized matrix; none-DM, PCL scaffold; BMSC, bone marrow stromal cell; WTO, wild-type osteocytes; daCO, osteocyte with dominant active β-catenin.