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. 2022 Aug 16;21:110–128. doi: 10.1016/j.bioactmat.2022.07.017

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7 — Angiogenesis of daCO-DM modified 3D-printed module. (a) At 8-week, HE staining to analyze the morphology of blood vessels in four groups. Scale bar 20 μm. (b) Semi-quantitative analysis of blood vessels. number of blood vessels per tissue area (N.Vessel/T.Ar). (c) Gene expressions of Vegfa and Angpt1 after 14 days of BMSCs culturing on DM modifed scaffolds. (d) Immunofluorescence of CD31 and Endomucin showed H-type angiogenesis in vivo. Scale bar 20 μm. DM, decellularized matrix; none-DM, PCL; BMSC, bone marrow stromal cell; WTO, wild-type osteocytes; daCO, osteocyte with dominant active β-catenin. Sections with newly formed bone were measured for each mouse and 5 mice were analyzed for each group. Data were expressed as mean ± SD. *p < 0.05 v.s. none-DM;^#p < 0.05 v.s. BMSC-DM, ^$p < 0.05 v.s. WTO-DM by one way ANOVA.