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. 2022 Aug 12;13:863034. doi: 10.3389/fimmu.2022.863034

Figure 5.

Figure 5

Blocking IL-13/STAT6 signaling pathway could ameliorate wrap restraint stress associated colitis. RCM-1 (1.7 mg/kg) was injected to WRS mice to block STAT6 signaling and then 1% OXA was given to induce colitis. (A) Body weight changes of control, WRS, WRS + RMC-1 injection mice after OXA enema. (B, C) Colon length at day 1 after 1% OXA enema of control, WRS, and WRS + RMC-1 injection mice. (D, E) Representative HE staining [×20] and pathological score of inflamed colon tissue of control, WRS, WRS + RMC-1 injection mice. (F, G) qPCR of Il-6 and Tnf-α mRNA in colonic tissues in control, WRS, WRS + RMC-1 injection mice at day 1 after 1% OXA enema. (H) Immunoblot for ZO-1 and occludin in colonic tissue in control, WRS, WRS + RMC-1 injection mice at day 1 after 1% OXA enema. Data are representative of at least two independent experiments. Data are shown as mean ± SEM. *p <0.05; ***p <0.001; ****p <0.0001.