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. 2022 Aug 11;13:973224. doi: 10.3389/fimmu.2022.973224

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Copyright © 2022 Peng, Ed-Dra, Song, Elbediwi, Nambiar, Zhou and Yue

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical staining sections of tight junction and the relative genes expression. Immunohistochemical staining was used to identify the presence of tight junction proteins (A) claudin-1 and (B) ZO-1 in intestinal tissues, and RT-qPCR was used to detect (C, D) the relative mRNA expression of tight junction protein genes. The arrow in the figure refers to a part of the stained tight junction protein. a, b, c, d: indicated the same item with different letters differ significantly (P < 0.05).