Table 2.
Primers used to analyze gene expression by quantitative RT-PCR.
| Gene | Primer sequence (5′–3′) | Size (bp) | Annealing temperature(°C) | Reference | GenBank No. |
|---|---|---|---|---|---|
| Claudin-1 | F: CTGATTGCTTCCAACCAG | 140 | 59 | (41) | NM_001013611 |
| R: CAGGTCAAACAGAGGTACAAG | |||||
| ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC | 131 | 59 | (41) | XM_413773 |
| R: CTGTGGTTTCATGGCTGGATC | |||||
| Bax | F: GTGATGGCATGGGACATAGCTC | 90 | 59 | (42) | XM_015290060 |
| R: TGGCGTAGACCTTGCGGATAA | |||||
| IL-1β | F: CTGGGCATCAAGGGCTACAA | 131 | 60 | (43) | NM_204524 |
| R: CGGTAGAAGATGAAGCGGGT | |||||
| IL-18 | F: TAACAGATCAGGAGGTGAAATCT | 299 | 60 | (44) | / |
| R: AAGGCCAAGAACATTCCTTGTT | |||||
| GAPDH | F: TGGAGAAACCAGCCAAGTAT | 145 | 55 | (45) | NM_204305.1 |
| R: GCATCAAAGGTGGAGGAAT |