. 2022 Aug 25;12:14533. doi: 10.1038/s41598-022-18330-5

Table 2.

Effect of the dietary supplementation (6 kg/T) with glutamate and glutamine in different ratio on haematological parameters of piglets at 8 days post-weaning.

Item¹	Diet²						SEM	P-value	Contrasts
Item¹	CO	100Glu	75Glu + 25Gln	50Glu + 50Gln	25Glu + 75Gln	100Gln	SEM	Diet	Linear	Quadratic	CO vs AAs addition	100Glu vs mixed addition	100Gln vs mixed addition
RBC, M/µL	6.92	7.07	6.81	6.75	6.76	6.8	0.1	0.530	0.180	0.181	0.552	0.059	0.869
HGB g/dL	11.9	12	11.6	11.7	11.7	11.8	0.2	0.708	0.567	0.142	0.687	0.108	0.458
HCT, %	37.8	38.1	36.3	36.6	36.5	37.6	0.6	0.201	0.680	0.023	0.291	0.025	0.130
MCV, fl	54.8	54.3	53.3	54.4	54.1	55.4	0.6	0.326	0.156	0.172	0.499	0.604	0.049
MCH, pg	17.2	17.2	17.1	17.3	17.3	17.5	0.3	0.946	0.332	0.819	0.884	0.771	0.438
MCHC, g/dL	31.5	31.6	32.1	31.9	32	31.5	0.3	0.492	0.782	0.133	0.252	0.237	0.157
RDW, %	25.4	25.4	24.5	24.9	25.1	24.6	0.8	0.947	0.696	0.866	0.583	0.766	0.553
Platelets, K/µL	600	614	665	668	640	580	44	0.638	0.515	0.121	0.473	0.386	0.130
Leukocytes, K/µL	15.66	15.07	13.76	13.92	15.67	14.07	0.91	0.457	0.976	0.797	0.234	0.717	0.555
Neutrophils, K/µL	7.39	6.94	6.63	7.77	7.66	6.58	0.55	0.468	0.864	0.167	0.633	0.515	0.220
Lymphocytes, K/µL	7.46	7.3	6.4	5.47	7.1	6.85	0.57	0.136	0.912	0.069	0.752	0.139	0.427
Monocytes, K/µL	0.46	0.59	0.44	0.45	0.54	0.32	0.08	0.178	0.068	0.783	0.943	0.196	0.063
Eosinophils, K/µL	0.31	0.19	0.27	0.18	0.33	0.29	0.04	0.011	0.026	0.961	0.113	0.107	0.460
Basophils, K/µL	0.04	0.04	0.03	0.05	0.04	0.04	0.01	0.965	0.796	0.807	0.956	0.943	0.943
Neutrophils, %	47.35	46.16	47.71	54.8	47.5	45.87	2.36	0.075	0.918	0.012	0.675	0.159	0.131
Lymphocytes, %	47.14	48.12	46.44	39.66	46.85	49.29	2.53	0.104	0.738	0.012	0.695	0.195	0.092
Monocytes, %	3.16	4.22	3.56	3.71	3.3	2.39	0.55	0.291	0.028	0.599	0.643	0.268	0.075
Eosinophils, %	2.03	1.21	2.04	1.39	2.11	2.12	0.23	0.010	0.012	0.764	0.297	0.279	0.012
Basophils, %	0.32	0.29	0.27	0.4	0.24	0.33	0.07	0.718	0.815	0.839	0.822	0.910	0.724
ROM, mmol H₂O₂/L	24.07	21.75	21.07	22.63	24.12	22.57	1.29	0.487	0.260	0.703	0.236	0.564	0.980
GPX-1, ng/mL	557	640	609	545	558	522	61.42	0.727	0.321	0.434	0.775	0.301	0.478
GSH, uM	0.31	0.14	0.39	0.18	0.21	0.17	0.11	0.640	0.439	0.810	0.449	0.377	0.495

¹RBC, red blood cells, HGB, haemoglobin, HCT, haematocrit, MCV, mean corpuscular volume, MCH, mean corpuscular haemoglobin, MCHC, mean corpuscular haemoglobin concentration, RDW, red cell distribution width.

Diet² CO = standard diet; 100Glu = CO plus 6 kg/Ton Glu; 75Glu + 25Gln = CO plus 4.5 kg/Ton Glu and 1.5 kg/Ton Gln; 50Glu + 50Gln = CO plus 3 kg/Ton Glu plus 3 kg/Ton Gln; 25Glu + 75Gln = CO plus 1.5 kg/Ton Glu and 4.5 kg/Ton Gln; 100Gln = CO plus 6 kg/Ton Gln. Significant values are in bold.