Table 3.

Effect of the dietary supplementation (6 kg/T) with glutamate and glutamine in different ratio on microbial taxa composition of piglets at 8- and 21-days post-weaning.

Comparison	Base Mean1	log2 Fold Change2	lfcSE3	P Value4	Padj5	Taxa
Day 8
Family level
100Gln vs CO	22.87	− 21.51	2.96	< 0.0001	< 0.0001	Fusobacteriaceae
Mixed additions vs Glu	10.78	21.06	3.41	< 0.0001	< 0.0001	Enterococcaceae
	1989.71	2.75	0.63	< 0.0001	< 0.0001	Lactobacillaceae
	7931.63	− 2.77	0.84	0.001	0.02	Erysipelotrichaceae
Mixed additions vs Gln	25.76	23.38	2.14	< 0.0001	< 0.0001	Fusobacteriaceae
	495.19	− 3.31	0.94	< 0.0001	0.001	Clostridiales_vadinBB60_group
Genus level
100Glu vs CO	19.62	− 21.65	2.78	< 0.0001	< 0.0001	Mogibacterium
	9.07	− 21.07	5.28	< 0.0002	0.007	Selenomonas
50Glu + 50Gln vs CO	6.7	− 18.56	3.91	< 0.0003	< 0.0001	Pelistega
	9.07	− 21.16	5.28	< 0.0004	0.006	Selenomonas
25Glu + 75 Gln vs CO	9.07	− 21.96	5.14	< 0.0005	0.004	Selenomonas
	740.85	− 3.7	1	< 0.0006	0.023	Blautia
100Gln vs CO	34.79	− 20.86	3.03	< 0.0007	< 0.0001	Fusobacterium
	8.33	− 21	3.12	< 0.0008	< 0.0001	UBA1819, Family Ruminococcaceae
Mixed additions vs Glu	57.99	23.33	2.92	< 0.0011	< 0.0001	Pediococcus
	11.38	21.04	3.41	< 0.0012	< 0.0001	Enterococcus
	1809.38	2.7	0.7	< 0.0013	0.008	Lactobacillus
Mixed additions vs Gln	37.65	24.02	2.21	< 0.0009	< 0.0001	Fusobacterium
	59.71	9.67	2.53	< 0.0010	0.013	Pediococcus
Day 21
Family level
100Glu vs CO	10.809	18.989	2.53	< 0.0001	< 0.0001	Fibrobacteraceae
75Glu + 25Gln vs CO	10.809	18.689	2.59	< 0.0001	< 0.0001	Fibrobacteraceae
50Glu + 50Gln vs CO	10.809	20.141	2.52	< 0.0001	< 0.0001	Fibrobacteraceae
25Glu + 75Gln vs CO	10.809	20.892	2.65	< 0.0001	< 0.0001	Fibrobacteraceae
100Gln vs CO	10.809	19.677	2.58	< 0.0001	< 0.0001	Fibrobacteraceae
	101.951	5.117	1.53	0.001	0.028	Bacteroidales_RF16_group
	109.322	2.14	0.68	0.002	0.037	Peptococcaceae
Genus level
100Glu vs CO	18.045	21.269	5.08	< 0.0001	0.006	Selenomonas_3
25Glu + 75Gln vs CO	18.045	22.13	5.35	< 0.0001	0.007	Selenomonas_3
CO vs AAs addition	7.378	− 21.523	4.13	< 0.0001	< 0.0001	Succiniclasticum

baseMean¹ = mean of normalized taxa counts averaged over all samples from both conditions. log2FoldChange² = log2 Fold Change. The sign is relative to the first group identified in the comparison. lfcSE³ = log2 Fold change standard error. P value⁴ = Wald statistic value. Padj⁵ = Benjamini–Hochberg adjusted p value.