Table 5.

Effect of the dietary supplementation (6 g/T) with glutamate and glutamine in the different ratios on the jejunal gene expression of piglets at 8 days post-weaning.

Gene1	Diet2						SEM	P-value	Contrasts
	CO	100Glu	75Glu  + 25Gln	50Glu  + 50Gln	25Glu  + 75Gln	100Gln		Diet	Linear	Quadratic	CO vs AAs addition	100Glu vs mixed addition	100Gln vs mixed addition
GLUL	1.17	1.78	1.38	1.64	1.55	1.48	0.21	0.409	0.501	0.251	0.088	0.338	0.834
OCLN	1.09	0.95	0.91	0.95	1.11	1.04	0.11	0.727	0.736	0.263	0.449	0.778	0.678
ZO-1	1.07	1.26	1.19	1.16	1.19	1.47	0.11	0.188	0.079	0.394	0.132	0.518	0.025
GPX-2	1.64	3.37	1.60	2.70	1.79	1.68	0.69	0.308	0.538	0.351	0.415	0.086	0.648
Myd88	1.02	1.01	0.99	1.16	0.96	0.97	0.07	0.331	0.704	0.338	0.977	0.727	0.418
TNF	1.11	1.06	1.37	0.98	0.94	0.90	0.15	0.359	0.175	0.364	0.728	0.849	0.296
MUC13	1.16	1.22	1.10	1.15	1.35	1.47	0.16	0.632	0.161	0.322	0.578	0.905	0.169
IL8	1.10	1.12	1.00	1.27	0.97	0.89	0.18	0.731	0.432	0.466	0.810	0.858	0.367
NFKB2	1.09	1.08	0.96	1.04	1.23	1.18	0.13	0.759	0.375	0.388	0.974	0.993	0.496
REG3G	1.00	1.01	0.36	0.90	0.85	2.25	0.48	0.925	0.407	0.887	0.382	0.811	0.586

Gene¹: Glutamate-Ammonia Ligase (GLUL); Occludin (OCLN); Tight Junction Protein 1 (ZO-1); Glutathione Peroxidase 2 (GPX-2); Innate Immune Signal Transduction Adaptor (MyD88); Tumor Necrosis Factor (TNF); Mucin 13 Cell Surface Associated (MUC13); C-X-C Motif Chemokine Ligand 8 (IL8/ CXCL8); Nuclear Factor Kappa B Subunit 2 (NFKB2); Regenerating Family Member 3 Gamma (REG3G). Diet² CO = standard diet; 100Glu = CO plus 6 kg/Ton Glu; 75Glu + 25Gln = CO plus 4.5 kg/Ton Glu and 1.5 kg/Ton Gln; 50Glu + 50Gln = CO plus 3 kg/Ton Glu plus 3 kg/Ton Gln; 25Glu + 75Gln = CO plus 1.5 kg/Ton Glu and 4.5 kg/Ton Gln; 100Gln = CO plus 6 kg/Ton Gln. Significant values are in bold.