Fig. 2.

Relative abundance of shared genus and species-level OTUs between the human stools and colonized mice are correlated post-fecal microbiota transfer (FMT). (a) Original human stool and 4% w/v inoculation slurry for each experiment prepared independently from human responder (R-FMT) and nonresponder (NR-FMT) stool. CS053, CS062, CS080 represent independent experiments. Species above 1% relative abundance are shown. (b) Taxonomic comparison at the genus level for human and mouse by Venn diagram and using Spearman correlation (graphs in lower panels). Cutoffs for inclusion: relative abundance > 0.01% and ubiquity > 34%. (c) Taxonomic comparison at the species/operational taxonomic unit (OTU) level for human and mouse by Venn diagram and using Spearman correlation (graphs in lower panels). The inclusion criteria were the same as genus level. (d) Heatmap with relative species abundances shown for donor human stool, inocula for individual experiments, and individual mice. Statistical comparisons were performed using Mann–Whitney U Test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data shown in B, C, and D include all mice used in the MC38 model across 3 independent experiments. + indicates OTUs referenced in manuscript