Fig. 4.

Species associated with MC38 tumor response in anti-PD-L1 treated R-FMT mice. C57BL/6 germ-free mice received human fecal microbiota transfers (FMT), followed by injection of MC38 tumor cells and treatment with the indicated isotype or anti-PD-L1 antibody. (a) R-FMT mice were categorized based on percent change in tumor volume: growth > 25% from day 10 were murine tumor progressors (MT-P, yellow, n = 15) and growth < 25% were murine tumor nonprogressors (MT-NP, blue, n = 13). (b) Bacterial species/OTUs enriched in MT-NP or MT-P. Statistical comparisons were performed using Mann–Whitney U Test: *p < 0.05, **p < 0.01. C. Correction for experimental variables (experiment, cage, and age) using a generalized linear model (GLM). OTUs above dotted line maintained significance after correction and are color coded based on direction of enrichment as shown in 4B. + indicates OTU label is truncated and full name is presented in Tables S3 and S4. Dotted line represents adjusted p < 0.05