TABLE 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| Pprof | 5′-TTTATAGGACTAAATATGACC-3′ |
| Placr | 5′-CAAGGCGATTAAGTTGGG-3′ |
| Phgpfus1 | 5′-ATTAGCATGAATTCCGAACACAACTATCCCATTC-3′ |
| Phgpnot1b | 5′-TTTGTTCTAAGCTTGCGGCCGCATTACTATTTTGGTTGG-3′ |
| Psdm1 | 5′-GCGGCCGCATAACGGAATAGGCAAGC-3′ |
| Psdm2 | 5′-TAACGGCAAGTGTTGCTTATGC-3′ |
| Plac1 | 5′-ATGTATTTGAATCTCCAATGAACATTGC-3′ |
| Plac2 | 5′-AACCGTTTAAATCTTGGATATGG-3′ |
a
Highlighted portions of sequence (by boldface type) are designed restriction sites in primers as follows: 5′-GAATTC-3′, EcoRI; 5′-AAGCTT-3′, HindIII; and 5′-GCGGCCGC-3′, NotI.
b
The primer Phgpnot1 contains both a HindIII and a NotI site.