TABLE 3.
Frequency of variable expression of the hgpA-lacZ translational gene fusion in H. influenzae HI1718
| Growth conditions, transition, expt, and parameter | No. of colonies and frequency of phenotypic transition
|
||
|---|---|---|---|
| CFU with phenotype change | Total CFU | Frequency (%) | |
| Heme-replete | |||
| Blue→White | |||
| Expt 1 | 76 | 5,976 | 1.27 |
| Expt 2 | 58 | 6,780 | 0.86 |
| Expt 3 | 194 | 11,096 | 1.75 |
| Expt 4 | 116 | 11,308 | 1.03 |
| Average (±SEM) | 1.23 (±0.19) | ||
| White→blue | |||
| Expt 1 | 99 | 16,316 | 0.61 |
| Expt 2 | 87 | 8,140 | 1.07 |
| Expt 3 | 63 | 7,612 | 0.83 |
| Expt 4 | 26 | 9,308 | 0.28 |
| Average (±SEM) | 0.70 (±0.17) | ||
| Heme-depleted | |||
| Blue→white | |||
| Expt 1 | 57 | 5,329 | 1.07 |
| Expt 2 | 33 | 4,176 | 0.79 |
| Expt 3 | 48 | 4,736 | 1.01 |
| Expt 4 | 52 | 3,777 | 1.38 |
| Average (±SEM) | 1.06 (±0.12) | ||
| White→blue | |||
| Expt 1 | 49 | 5,680 | 0.86 |
| Expt 2 | 17 | 4,364 | 0.39 |
| Expt 3 | 42 | 10,108 | 0.42 |
| Average (±SEM) | 0.56 (±0.15) | ||
| Hemoglobin-haptoglobin-passaged | |||
| Blue→white | |||
| Expt 1 | 103 | 10,010 | 1.03 |
| Expt 2 | 63 | 8,459 | 0.74 |
| Expt 3 | 74 | 7,756 | 0.95 |
| Average (±SEM) | 0.91 (±0.09) | ||
| White→blue | |||
| Expt 1 | 54 | 8,246 | 0.65 |
| Expt 2 | 160 | 21,489 | 0.74 |
| Expt 3 | 65 | 27,201 | 0.24 |
| Average (±SEM) | 0.54 (±0.15) | ||