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. 2021 Nov 29;16(3):397–419. doi: 10.1007/s12079-021-00646-y

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Fig. 5 — TSP1 regulates the subcellular localization of exportin-1 and RanBP1 in a CD47-dependent manner. a, b Confocal microscopic analysis of CD47/exportin-1 and exportin-1/RanBP1 in T cells treated with LMB and TSP1 (1 μg/ml) for 1 h. Scale bar = 5 μm. c Western blots with anti-exportin-1 and RanBP1 in cytosolic, nuclear, and EV fractions from untreated WT and CD47⁻ T cells and cells treated with LMB and TSP1. d–g Quantification of western blots by calculating the ratio of nuclear/cytosolic was determined by normalizing to immunoblots for GAPDH or CROP loading controls, respectively (n = 2)