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. 2021 Dec 1;16(3):377–395. doi: 10.1007/s12079-021-00659-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Time course of CCN2 and CCN5 mRNA levels in infarcted and non-ischemic myocardial tissue after induction of experimental myocardial infarction in mice. Histogram demonstrating CCN2 (panel a) and CCN5 (panel b) mRNA levels relative to 18S rRNA (house-keeping) in infarcted region and in non-ischemic myocardial tissue, harvested 2, 7, and 50 days after permanent ligation of the left coronary artery and normalized to levels in myocardial tissue of sham-operated mice. A column (open column) representing levels in myocardial tissue of sham-operated mice has been included both in front of columns showing levels in the infarcted region and in front of columns demonstrating levels in non-ischemic myocardial tissue. mRNA levels were analyzed by real-time RT-PCR. Data are shown as the mean ± SEM (n = 3 in each group). The data were subjected to statistical analyses by one-way ANOVA with Dunnett’s post hoc test. Statistically significant alterations were indicated by **p < 0.01; ***p < 0.001; ****p < 0.0001 versus sham-operated mice (SHAM); ns: indicates not statistically significant versus sham-operated mice (SHAM)