TABLE 3.
Support of bacteriophage growth by MGM100(pHsp60-Hsp10) at 37°Ca
| Plasmid | Relative numbers of plaques with phage (medium)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| λv (LB)
|
λv (BBL)
|
T4 (LB)
|
T4 (BBL)
|
T5 (LB)
|
||||||
| Ara | Glc | Ara | Glc | Ara | Glc | Ara | Glc | Ara | Glc | |
| pBR325 | 1* | 1.1 | 1.0 | 1* | 1 | |||||
| pHsp60-Hsp10 | 0.9 | <2 × 10−3 | 1.1 | 0.7 | 0.8 | <2 × 10−3 | NT | <2 × 10−3 | 0.6 | 0.5 |
| pGroES-GroEL | 1.0 | 0.8 | 1.1 | 1.0 | 0.4 | 0.9 | NT | 1.1 | 0.9 | 0.6 |
a
MGM100 containing the indicated plasmid and grown for plating on LB plus ampicillin (50 μg/ml) plus 0.2% arabinose or, where it would support growth, 0.2% glucose was used as described. Numbers of plaques varied between 200 and 800 per plate; italicized numbers indicate that plaques were small. T4 and λ experiments were repeated several times and normalized, respectively, to values labelled (*). NT, not tested.