Fig. 2. Overexpression of lncRNA HEPFAL inhibits cell colony formation, migration, and invasion in vitro.
A, B Relative expression of lncRNA HEPFAL and SLC7A11 in 6 different liver cancer cell lines was detected by qRT-PCR. C The expression of SLC7A11 in 6 different liver cancer cell lines was detected by western blot. D, E, G, H Relative expression of lncRNA HEPFAL and SLC7A11 in HCCLM3 and PLC cells transfected with lncRNA HEPFAL expression vector and control vector, respectively. F, I The expression of SLC7A11 and GPX4 in HCCLM3 and PLC cells transfected with lncRNA HEPFAL expression vector and control vector were detected by western blot. J Cell migration capacities were conducted by wound healing assays after transfection. K Cell migration and invasion abilities were indicated by transwell assays in each group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.