Figure 1.

Effects of P2RY₁₂ on PAF and LPS-induced NET release and inflammatory responses. (A) Different concentrations of PAF or LPS induced the release of cfDNA from WT or P2RY₁₂-KO neutrophils (n = 4). (B) Immunofluorescent staining and statistical analysis of CitH3 and DAPI staining of WT or P2RY₁₂-KO neutrophils induced by PAF and LPS, respectively (n = 4). (C, D) Release of IL-6, IL-1β, and TNF-α in medium of WT or P2RY₁₂-KO neutrophils induced by PAF and LPS, respectively (n = 4). All the data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT control; ^### P < 0.001 vs. P2RY₁₂-KO Control; ^& P < 0.05, ^&& P < 0.01, ^&&& P < 0.001, WT vs. P2RY₁₂-KO. #P < 0.05, ##P < 0.01.