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. 2022 Aug 12;13:918476. doi: 10.3389/fimmu.2022.918476

Figure 6.

Figure 6

Effects of Rg5 on PAF or LPS-induced activation of PAD4 and ERK/NF-κB signaling pathway in WT or P2RY12-KO neutrophils. (A) Calcium flux in WT or P2RY12-KO neutrophils stimulated by PAF (n = 3). (B) Protein expression of PAD4 in WT or P2RY12-KO neutrophils stimulated by PAF (n = 5). (C) Activity of PAD4 in WT or P2RY12-KO neutrophils stimulated by PAF (n = 4). (D, E) Western Blot results and statistical analysis of protein expression and phosphorylation levels of ERK (n = 5) and NF-κB (n = 5) in WT or P2RY12-KO neutrophils stimulated by PAF or LPS. All the data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. PAF or LPS group.