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. 2022 Aug 12;13:754271. doi: 10.3389/fphar.2022.754271

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Copyright © 2022 Zhu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Urokinase inhibits H/R-induced cell death and cytokine secretion. An in vitro model of APE in BEAS-2B cells. Cells were subjected to hypoxic conditions for 12 h followed by reoxygenation for 12 h (for details refer to Materials and Methods). (A) Representative images from terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assay. (B) Graph shows quantification of images in (A). Data are represented as mean ± SD; ****p < 0.0001, ns, not significant (n = 5). (C) Secretion of indicated cytokines in the cell supernatant was determined using Luminex assay. represented as mean ± SD; ****p < 0.0001, ns, not significant (n = 3) (UK: urokinase).