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. 2022 Aug 12;13:754271. doi: 10.3389/fphar.2022.754271

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Copyright © 2022 Zhu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyubiquitination of uPAR occurs via lysine 48-linked chains. (A) Lysates obtained from BEAS-2B cells subjected with or without H/R ± MG-132 were immunoprecipitated using an anti-uPAR antibody. To confirm successful ubiquitination, blots were probed with an anti-Ub antibody to determine poly-ubiquitination and anti-uPAR antibodies. (B) BEAS-2B cells subjected to H/R for indicated time points underwent MG-132; western blotting was done with anti-uPAR. (C) Same as (B), but the lysates were immunoprecipitated using anti-uPAR and detected with anti-lysine 48K-Ub. Shown are representative blots from three independent experiments. The results showed that uPAR is poly-ubiquitinated via lysine 48-linked chains in BEAS-2B cells subjected to H/R.