Table 1.
Determination methods of tetracycline antibiotics residues in milk samples by HPLC-DAD.
| Compound(s) | Stationary phase | Mobile phase | Detection | Reference |
|---|---|---|---|---|
| TTR | C18 column | Isocratic elution: 0.01 M oxalic acid, ACN and MeOH (70:20:10 v/v/v) | HPLC-DAD at 385 and 276 nm | 29 |
| OXY, TTR *The injection volume was 75 µL | C18 column | Isocratic elution: 0.05 M potassium dihydrogen phosphate (pH 2.8) and ACN (80:20, v/v) | HPLC-UV at 232 nm | 26 |
| TTR, OXY, MNC, CTC, MTC and DC | C18 column | Gradient elution: ACN and NaH2PO4 | HPLC-DAD at 350 nm | 30 |
| TTR, 4-epi TC and OXY | C18 column | Isocratic elution: 0.010 M Oxalic acid:ACN:MeOH (150:20:20 v/v/v) | HPLC-DAD at 365 and 280 nm | 31 |
| TTR, OXY, CTC and DC | C18 column | Gradient elution: 0.01 M Oxalic acid and ACN | HPLC-DAD at 360 nm | 32 |
| OXY, TTR, epi-CTC, CTC and DC | C18 column | Gradient elution: 0.01 M Oxalic acid and ACN | HPLC-DAD at 355 nm | 33 |
| OXY, TTR and CTC | C18 column | Isocratic elution: 0.01 M potassium dihydrogen phosphate:ACN:MeOH (70:20:10 v/v/v), pH 4 | HPLC-DAD at 358 nm | This work |
OXY Oxytetracycline, TTR tetracycline, CTC Chlortetracycline, MTC Methacycline, MNC Minocycline, DC Doxycycline, 4-epiTC 4-epiTetracycline, epi-CTC epi-Chlortetracycline, ACN Acetonitrile, MeOH Methanol.