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. 2022 Aug 25;13:5002. doi: 10.1038/s41467-022-32686-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The phalloidin staining of Huh-7 cells with and without the treatment of various nanofilaments. Scale bars represent 20 μm. b The spreading area and the perimeter area ratio of HuH-7 cells under various conditions. Kruskal-Wallis with Dunn’s multiple comparisons test was used for analysis of the data. Data are presented as mean ± s.d. From left to right, n = 61, 49, 45, 46, 51, 56 cells, respectively. The trajectory plots c, and the correlated quantitative analysis of travel speed, persistence and persistence index d of randomly selected migrating cells for each incubation condition. Live cell images were taken every 10 min for a total of 10 h. Kruskal-Wallis with Dunn’s multiple comparisons test was applied in data analysis. Error bars represent standard error of mean. From left to right, n = 261, 280, 230, 260, 214, and 278 cells, respectively. Scale bars in panel c represent 50 μm. Source numerical data are available in source data.