Table 2.
Top 10 genes by integration frequency
| Gene | Number of integration sites | Percent of integration sites | Number of reads | Percent of reads | FAH homology (nucleotides/percent) | |
|---|---|---|---|---|---|---|
| No. 162 | ZNF24 | 12 | 1.319 | 29698 | 29.991 | 11/100% |
| MIR17 | 14 | 1.538 | 17995 | 18.172 | 7/100% | |
| MIR92A-1 | 4 | 0.440 | 4128 | 4.169 | none | |
| TRPC4 | 5 | 0.549 | 3088 | 3.118 | 11/100% | |
| TTR | 3 | 0.330 | 2266 | 2.288 | 12/92% | |
| PRMT6 | 7 | 0.769 | 1954 | 1.973 | 12/92% | |
| HSP90AB1 | 1 | 0.110 | 1663 | 1.679 | 11/100% | |
| FAM107A | 4 | 0.440 | 1290 | 1.303 | 24/83% | |
| NCOA1 | 2 | 0.220 | 1231 | 1.243 | 11/100% | |
| IFI44 | 3 | 0.330 | 1202 | 1.214 | 17/82% | |
| No. 166 | ACVR2A | 12 | 0.288 | 468183 | 8.104 | 9/100% |
| VGLL3 | 46 | 1.103 | 346816 | 6.003 | 10/100% | |
| UOX | 27 | 0.647 | 282277 | 4.886 | 9/100% | |
| MYC | 4 | 0.096 | 240240 | 4.158 | 13/92% | |
| ITGA2 | 98 | 2.350 | 189860 | 3.286 | 11/100% | |
| MIR7135 | 14 | 0.336 | 178740 | 3.094 | 28/82% | |
| ZRANB2 | 77 | 1.847 | 166959 | 2.890 | 39/74% | |
| AKTIP | 13 | 0.312 | 163213 | 2.825 | 11/100% | |
| PNPT1 | 17 | 0.408 | 159257 | 2.757 | 13/92% | |
| MGAT4C | 24 | 0.576 | 100552 | 1.740 | 17/88% | |
| No. 167 | DPP4 | 53 | 2.340 | 93939 | 3.649 | 14/93% |
| ASB3 | 31 | 1.369 | 83777 | 3.255 | 14/86% | |
| SH3GLB1 | 6 | 0.265 | 78216 | 3.038 | 19/79% | |
| DPH5 | 4 | 0.177 | 78209 | 3.038 | 16/94% | |
| RSPO3 | 8 | 0.353 | 77394 | 3.007 | 10/100% | |
| ITGAV | 6 | 0.265 | 75559 | 2.935 | 15/87% | |
| CTSB | 22 | 0.971 | 59593 | 2.315 | 18/83% | |
| CCL28 | 50 | 2.208 | 55120 | 2.141 | 11/100% | |
| DDX58 | 21 | 0.927 | 54736 | 2.126 | 21/81% | |
| MGAT4C | 5 | 0.221 | 52320 | 2.032 | 17/88% |
LV-FAH integration sites were evaluated from representative sections of liver from pigs Nos. 162, 166, and 167. Data are reported by the percent of reads each individual gene represented of the total for that animal/tissue. The number of unique integration sites within a given gene, and the percent of unique integrations sites that represents of the total for each tissue/animal is also presented for context. Finally, the region of highest homology to FAH is presented for each. MGAT4C, included in the top ten genes with highest relative integration frequency for both pigs Nos. 166 and 167, is a transferase essential to production of some N-linked sugar chains.