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. 2022 Aug 12;9:890653. doi: 10.3389/fmolb.2022.890653

FIGURE 1.

FIGURE 1

Effect of omaveloxolone on cell growth, ATP production, ROS production, MMP, and mitochondrial and mtDNA content. An equal amount of control (CONT) cells with mitochondrial CI (C1) or COX (COX) deficiency or PD with mutated PRKN (PD-P) or mutated DJ-1 (PD-D) was seeded in microtiter wells, cultivated in the presence or absence of 50 nM omaveloxolone (OMA) for 72 h in permissive (GLU) or restrictive (GAL) medium where after the following parameters were measured; (A) growth ratio GAL vs. GLU measured by methylene blue staining (MB); (B) mitochondrial ATP production measured by luciferin–luciferase (RLU) normalized to MB; (C) ROS production measured by DCF and normalized to MB; (D) mitochondrial membrane potential (MMP) was calculated with the ratio of TMRE to MTG relative fluorescence units (RFU); (E) mitochondrial content was measured by MTG, RFU and normalized to MB. (F) mtDNA content was measured by qPCR of the mitochondrial MT-TL1gene and normalized to the nuclear β2M. Results are presented as mean ± SD relative to controls (n = 3). #p < 0.05 compared to untreated control mean, *<0.05 compared to the individual cell without additive.