Fig. 5.

Mean fluorescence enhancements of SSP4 (5 μM) with 25 μM S₈ in the presence of (A) various analytes: (1) control; (2) 1 mM GSH; (3) 200 μΜ Ηcy; (4) 30 μM methionine (Met); (5) 100 μM Cys; (6) 100 μM GSH; (7) 100 μM N-acetyl-l-cysteine (NAC); (8) 150 μM glycine (Gly); (9) 55 μM tyrosine (Tyr); (10) 40 μM tryptophan (Trp), (11) 100 μM arginine (Arg). (B) metal ions: 1) control; (2) CuSO₄; (3) FeCl₂; (4) FeCl₃; (5) MgCl₂; (6) CaCl₂; (7) ZnCl₂ at 20 equivalents (500 μΜ) to the 25 μM S₈. Results are expressed as mean ± SEM (n = 3). Statistical analyses were performed using one-way ANOVA. ****P < 0.0001, *P = 0.0326–0.0431, ns = not significant.