Fig. 8.

Representative cell images with SSP4. (I) Brightfield (A, D), fluorescence (B, E), and overlaid images (C, F) of Η₂S₂ in HeLa cells. Cells were first treated with 5 μΜ of SSP4, then washed and subjected to (A–C) No Na₂S₂ added, (D–F) 50 μM Na₂S₂. (II) COS-7 cells overexpressing CSE and WT and (III)CARS2 KO HEK293T were subjected to fluorescence staining. Fluorescence images (left panel) and fluorescence intensities (right panel) of SSP4 staining. Scale bars, 100 μm. Data are mean values ± S.D (from three different images, each containing more than 100 cells in a representative experiment). *P < 0.05, **P < 0.01; Student's t-test.