Fig. 2.

GSH and Ca²⁺induce the formation of highly unstable forms of TTR Samples of TTR (2.3 μM) in Tris buffer were supplemented as indicated with 100 mM GSH and 200 mM CaCl₂ at final concentrations. Samples, prepared in quintuplicate, were incubated at room temperature, and the fluorescence emission spectra were recorded after the indicated periods of time for each sample. The spectra obtained using excitation of 360 nm (A), 275 nm (B) and 315 nm (C) at all incubation times for each sample were superimposed.