Fig. 3.

GSH and Ca²⁺induce the formation of dbAF and dityrosine in substoichiometric populations of TTR Samples of TTR (4.1 μM) in Tris buffer were supplemented as indicated with 2 mM GSH and 200 mM CaCl₂ at final concentrations. Samples were incubated at room temperature, and for each sample, the fluorescence emission spectra were recorded after the indicated periods of time. The spectra obtained for all samples using excitation of 360 nm (A), 275 nm (B) and 315 nm (C) were superimposed for each incubation time. The same sample was used for spectra recording after each incubation.