TABLE 2.

Bond cleavage frequencies and specific activities of the E. chrysanthemi 3937 endopectate lyases PelA, PelI, PelL, PelD, and PelB acting on oligogalacturonates of defined lengths^a

Enzyme	nb	Oligogalacturonatec	Activity (U · mg−1)
PelA	3	G — G — G	0.6
		100
	4	G — G — G — G	17
		100
	5	G — G — G — G — G	12
		23    77
	6	G — G — G — G — G — G	15
		33     6    61
	7	G — G — G — G — G — G — G	23
		70     9     2    19
	8	G — G — G — G — G — G — G — G	25
		62    19     6     2    11
PelI	3	G — G — G	0.6
		100
	4	G — G — G — G	11
		62    38
	5	G — G — G — G — G	87
		43    22    34
	6	G — G — G — G — G — G	252
		74    23     3
	7	G — G — G — G — G — G — G	504
		4    91   5
	8	G — G — G — G — G — G — G — G	630
		2    33    61   4
PelL	3	G — G — G	0
	4	G — G — G — G	0.02
		100
	5	G — G — G — G — G	0.22
		28    72
	6	G — G — G — G — G — G	2.2
		64    34     2
	7	G — G — G — G — G — G — G	3.5
		4    91    5
	8	G — G — G — G — G — G — G — G	3
		3   38   57   2
PelD	3	G — G — G	13
		100
	4	G — G — G — G	418
		100
	5	G — G — G — G — G	310
		8    92
	6	G — G — G — G — G — G	268
		3     3    94
	7	G — G — G — G — G — G — G	250
		4     7    16    73
	8	G — G — G — G — G — G — G — G	222
		1    14    10    75
PelB	3	G — G — G	40
		100
	4	G — G — G — G	2,400
		43    57
	5	G — G — G — G — G	3,660
		69    31
	6	G — G — G — G — G — G	4,070
		10    52    38
	7	G — G — G — G — G — G — G	3,780
		2     6    62    30
	8	G — G — G — G — G — G — G — G	3,160
		11    67    22

^aConditions of this experiment included the following: 0.5 mM oligogalacturonate (n = 3 to 8), 1 mM CaCl₂, and 20 mM Tris-HCl (pH 8.0) (for PelA, PelD, and PelL) or 20 mM amino-methyl-propanol–HCl (pH 9.0) (for PelB and PelI) at 37°C. Product analysis was done as described in Materials and Methods. 

^bn, degree of polymerization. 

^cBond cleavage frequencies are given in percentages. The reducing ends of the oligogalacturonates are printed in bold type.