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. 2022 Aug 12;9:940449. doi: 10.3389/fmolb.2022.940449

FIGURE 2.

FIGURE 2

UBC9 interaction with E-cadherin and its transcriptional regulation. (A) HPV-positive 93VU147T and HPV-negative UMSCC10A cell lines were transduced with an inducible shUBC9 lentiviral vector, and treated or not with doxycycline for 72 h. Cells were then harvested and whole cell extracts were subjected to anti-UBC9 immunoprecipitation (IP). Immunoblotting with the indicated antibodies was then performed and whole cell extracts were used as input controls. (B) Phase-contrast pictures of scratch wound healing assay performed on HPV-positive 93VU147T and HPV-negative UMSCC19, transfected for 72 h with SUMO1 or LUC siRNA at time 0 and 18 h after the scratch. (C) Graph represented the percentage of wound closure (means ± SEM) of SUMO1 siRNA cells as compared with LUC siRNA transfected; ****, p < 0.0001, ns, not significant (unpaired t test). (D) HPV-positive HNC cell lines (UMSCC47, UPCISCC154, 93VU147T) and HPV-negative (UMSCC10A, UMSCC19, UMSCC23) cell lines were transfected for 72 h with specific siRNA against SUMO1 or Luciferase as control. After transfection, total RNAs were isolated for RT-qPCR and reported as means ± SEM of fold changes of at least three independent experiments; *, p < 0.05, ns, not significant (unpaired t test).