Table 2. Oligonucleotide primers used in amplification of the capripoxvirus by conventional polymerase chain reactions.
Protein coding gene | Oligonucleotides | Positions | Length of amplicon (bp) |
---|---|---|---|
GPCR | Forward: ATGAATTATACTCTTAGCACAGTT | 8511–7366 | 1,146 |
Reverse: TTATCCAATGCTAATACTAC | |||
RPO30 | Forward: ATGGATGATGATAATACTAATTCA | 28973–28368 | 606 |
Reverse: TTATTTTTCTACAGCTCTAAAC | |||
P32 | Forward: ATGGCAGATATTCCATTATATGTT | 65361–66329 | 969 |
Reverse: CTAAATTATATACGTAAATAACA |