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Expression of ECM-associated proteins in TGF-β-stimulated colonic fibroblasts CCD-18Co. CCD-18Co cells were incubated with or without TGF-β (10 ng/mL) for 24 h. α-SMA protein level (A) was studied by Western Blot. GAPDH expression served as the loading control for the amount of protein. mRNA levels of CTGF (B), COL1A1 (C) and COL3A1 (D) were studied by RT-qPCR. * means p < 0.05 vs. Ctrl. Results are expressed as mean ± SEM of four independent experiments.
