Figure 2.

Effect of increasing concentrations of FICZ from 10 to 100 nM on fibrosis markers in TGF-β-stimulated colonic fibroblasts CCD-18Co. CCD-18Co cells were simultaneously stimulated with TGF-β (10 ng/mL) and FICZ (0 (CT); 10 (F10); 100 (F100); 1000 (F1000) nM) for 24 h. α-SMA protein level (A) was studied by Western Blot. GAPDH expression serves as the loading control for the amount of protein. mRNA levels of CTGF (B), COL1A1 (C), COL3A1 (D) AhR (E) and ARNT (F) were studied by RT-qPCR. * means p < 0.05. Results are expressed as mean ± SEM of four independent experiments.